BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10001

Title: A high resolution structure of mastoparan-X strongly bound to lipid-bilayer membrane determined by solid-state NMR   PubMed: 16714348

Authors: Akutsu, Hideo; Fujiwara, Toshimichi; Todokoro, Yasuto

Citation: Todokoro, Yasuto; Yumen, Ikuko; Fukushima, Kei; Kang, S.-W.; Park, J.-S.; Kohno, Toshiyuki; Wakamatsu, Kaori; Akutsu, Hideo; Fujiwara, Toshimichi. "Structure of Tightly Membrane-Bound Mastoparan-X, a G-protein-Activating Peptide, Determined by Solid-State NMR"  Biophys. J. 91, 1368-1379 (2006).

Assembly members:
MP-X, polymer, 14 residues, 1556 Da.

Natural source:   Common Name: wasps   Taxonomy ID: 7448   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Vespa simillima

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MP-X: INWKGIAAMAKKLX

Data sets:
Data typeCount
13C chemical shifts73
15N chemical shifts20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MP-X1

Entities:

Entity 1, MP-X 14 residues - 1556 Da.

1   ILEASNTRPLYSGLYILEALAALAMETALA
2   LYSLYSLEUNLW

Samples:

sample_1: mastoparan-X, [U-95% 13C; U-95% 15N], 5 mg; liposome 50 mg

condition_1: pH: 7.4; temperature: 253 K

condition_2: pH: 7.4; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
3D N-CA-COsample_1isotropiccondition_1
3D Ni-COi+1-CAi+1sample_1isotropiccondition_1
2D RFDRsample_1isotropiccondition_1
2D SPCz5sample_1isotropiccondition_1
2D (CACB)i+1-(COCA)isample_1isotropiccondition_2
2D Ni-(COCA)i+1sample_1isotropiccondition_2
2D N-CAsample_1isotropiccondition_2
2D Ni-(COCACB)i+1sample_1isotropiccondition_1

Software:

Felix2000 v2000, Accelrys Inc. - assignment

spinsight v4.3.2, VARIAN - processing

NMR spectrometers:

  • Varian CMX 600 MHz
  • Varian CMX 500 MHz

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